Yubikey guide for Git Signing, SSH Auth, U2F 2FA, and 1Password (2017)
After a little setup, an engineer inserts their Yubikey, enters a PIN, and then their SSH key is loaded all the time the device is connected. Yubikeys support U2F, which makes 2FA as simple as pressing the button on your Yubikey device. This is a great way to get your (very long, very hard to remember) 1Password secret key when using a shared machine.
Source: www.engineerbetter.com